Rubinstein C, Jone C, Trosko J E, Chang C C
Fundam Appl Toxicol. 1984 Oct;4(5):731-9. doi: 10.1016/0272-0590(84)90094-0.
Using the Chinese hamster V79 in vitro cell system designed to measure intercellular communication, 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), and several mixtures of these compounds were tested for their ability to inhibit this biological process. The ability of these chemicals to inhibit colony-forming ability of these cells was tested prior to the studies to measure intercellular communication. 2,4-D was less cytotoxic than 2,4,5-T. Both 2,4,5-T and 2,4-D were able to inhibit intercellular communication at their respective noncytotoxic dose ranges. Various mixtures of both chemicals were also able to inhibit intercellular communication, showing some kind of additivity. No-effect levels were also noted in the intercellular communication assay. These results were interpreted as being consistent with the hypothesis that these compounds might be teratogenic by their ability to inhibit intercellular communication during development.
利用旨在测量细胞间通讯的中国仓鼠V79体外细胞系统，对2,4 - 二氯苯氧乙酸（2,4 - D）、2,4,5 - 三氯苯氧乙酸（2,4,5 - T）以及这些化合物的几种混合物抑制这一生物学过程的能力进行了测试。在进行测量细胞间通讯的研究之前，先测试了这些化学物质抑制这些细胞集落形成能力的情况。2,4 - D的细胞毒性低于2,4,5 - T。2,4,5 - T和2,4 - D在各自的非细胞毒性剂量范围内均能抑制细胞间通讯。两种化学物质的各种混合物也能抑制细胞间通讯，呈现出某种加和性。在细胞间通讯试验中也观察到了无效应水平。这些结果被解释为与以下假设一致，即这些化合物可能因其在发育过程中抑制细胞间通讯的能力而具有致畸性。
