Brufani M, Lippa S, Littarru G P, Oradei A, Pomponi M
Ital J Biochem. 1984 Sep-Oct;33(5):325-32.
The authors, with a spectrophotometric procedure, have evaluated the kinetic constants of physostigmine, a cholinesterase inhibitor which assumes an important function when the degeneration of cholinergic projections is observed. This approach overcomes some of the difficulties encountered in measuring the binding and the carbamylation rate constants. A kinetic treatment, consistent with the experimental results observed in the enzyme-inhibitor system in the presence of substrate, is developed.
作者们采用分光光度法评估了毒扁豆碱的动力学常数，毒扁豆碱是一种胆碱酯酶抑制剂，在观察到胆碱能投射退化时发挥重要作用。这种方法克服了在测量结合常数和氨甲酰化速率常数时遇到的一些困难。基于在有底物存在的酶 - 抑制剂系统中观察到的实验结果，开展了一种动力学处理方法。
