McGarrity G J, Kotani H
Isr J Med Sci. 1984 Oct;20(10):924-6.
Invertebrate and mammalian cell cultures have been utilized to study various properties of spiroplasmas. Recent studies have focused on the interaction of Spiroplasma mirum with AG-4676, a cell line derived from the rat eye lens. S. mirum produced cytotoxicity in this cell line, consisting of vacuolization and an increase in the number of binucleated cells. The number of S. mirum organisms attached to the cell increased with increased age of infection. S. mirum does not grow in Dulbecco's modified Earle's medium + 10% fetal bovine serum, the growth medium for AG-4676. It does, however, grow in conditioned medium obtained as supernatant of 1- to 5-day-old AG-4676. This conditioned medium from AG-4676 also supported growth of S. mirum (GT-48), corn stunt spiroplasmas (Rio Grande) and the following unspeciated strains: M-55 (I-6), N-525 (I-7), CN-5 (IX) and AES-1 (X). It did not support S. citri, honeybee BC-3, EA-1 (VIII), MQ-6 (XII) or CC-1 (XIII). The growth promotion titer for S. mirum was 1:8, but this varied with the lot of fetal bovine serum. Biochemical characterization of this growth-promoting factor is in progress.
无脊椎动物和哺乳动物细胞培养已被用于研究螺旋体的各种特性。最近的研究集中在微小螺旋体与AG - 4676（一种源自大鼠眼晶状体的细胞系）之间的相互作用。微小螺旋体在该细胞系中产生细胞毒性，表现为空泡化和双核细胞数量增加。附着在细胞上的微小螺旋体数量随着感染时间的延长而增加。微小螺旋体不能在杜氏改良伊格尔培养基+10%胎牛血清（AG - 4676的生长培养基）中生长。然而，它能在1至5日龄AG - 4676的上清液作为条件培养基中生长。这种来自AG - 4676的条件培养基也支持微小螺旋体（GT - 48）、玉米矮化螺旋体（里奥格兰德）以及以下未分类菌株的生长：M - 55（I - 6）、N - 525（I - 7）、CN - 5（IX）和AES - 1（X）。它不支持柑桔螺旋体、蜜蜂BC - 3、EA - 1（VIII）、MQ - 6（XII）或CC - 1（XIII）。微小螺旋体的生长促进效价为1:8，但这会因胎牛血清的批次不同而有所变化。这种生长促进因子的生化特性研究正在进行中。
