Chang C J, Chen T A
Yale J Biol Med. 1983 Sep-Dec;56(5-6):439-45.
Substrate utilization in defined media for two flower spiroplasmas (S. floricola and FS SR-3) and honeybee spiroplasma (HBS AS-576) was investigated. Glucose, fructose, and mannose were utilized by all three spiroplasmas. In addition, HBS (AS-576) could ferment trehalose; FS (SR-3), sucrose; and S. floricola, trehalose, sucrose, and raffinose. The three spiroplasmas varied greatly in growth requirements for amino acids. Only S. floricola utilized arginine. HBS (AS-576) required at least one purine and one pyrimidine base for growth, while both flower spiroplasmas grew with only one base in the medium. Oleic acid, cholesterol, and BSA were essential to all three spiroplasmas. Palmitic acid, which was non-essential, promoted growth significantly.
研究了在特定培养基中两种花卉螺原体(弗洛里科拉螺原体和FS SR-3)以及蜜蜂螺原体(HBS AS-576)的底物利用情况。所有三种螺原体都能利用葡萄糖、果糖和甘露糖。此外,HBS(AS-576)可发酵海藻糖;FS(SR-3)可发酵蔗糖;弗洛里科拉螺原体可发酵海藻糖、蔗糖和棉子糖。这三种螺原体对氨基酸的生长需求差异很大。只有弗洛里科拉螺原体利用精氨酸。HBS(AS-576)生长需要至少一种嘌呤碱和一种嘧啶碱,而两种花卉螺原体在培养基中仅有一种碱基时就能生长。油酸、胆固醇和牛血清白蛋白对所有三种螺原体都是必需的。非必需的棕榈酸能显著促进生长。