Assink H A, Blijenberg B G, Boerma G J, Leijnse B
J Clin Chem Clin Biochem. 1984 Oct;22(10):685-92. doi: 10.1515/cclm.1984.22.10.685.
The bromocresol purple methods for the determination of serum albumin on the Technicon SMAC and the DuPont ACA systems were evaluated and compared with the corresponding bromocresol green methods, a radial immunodiffusion (RID) and an electrophoresis technique. The linearity and precision of the dye binding methods were comparable. The bromocresol purple methods were more influenced by lipaemia and to a lesser extent by high bilirubin concentrations. The specificity of the bromocresol purple methods was superior to that of the bromocresol green methods and showed excellent correlations with the radial immunodiffusion method: bromocresol purple: SMAC = 1.03 (RID) + 0.32; bromocresol purple: ACA = 1.00 (RID) + 1.27. The bromocresol purple methods intercorrelated better than the bromocresol green methods. Freeze-dried sera can be used for albumin standardization. Analysis of the albumin fractions of freeze-dried and liquid sera by high-performance liquid chromatography (HPLC) did not reveal significant differences in albumin polymerization. Purified albumin reacted, irrespective of the degree of polymerization, very differently in the dye-binding methods. The bromocresol purple methods showed an overestimation of purified albumin, the bromocresol green methods an underestimation. The reason for these differences is unclear. Non-human albumin reacted poorly with bromocresol purple in contrast with bromocresol green.
对Technicon SMAC和杜邦ACA系统上用于测定血清白蛋白的溴甲酚紫方法进行了评估，并与相应的溴甲酚绿方法、放射免疫扩散（RID）法和电泳技术进行了比较。染料结合法的线性和精密度相当。溴甲酚紫方法受脂血的影响更大，受高胆红素浓度的影响较小。溴甲酚紫方法的特异性优于溴甲酚绿方法，并且与放射免疫扩散法显示出良好的相关性：溴甲酚紫：SMAC = 1.03（RID）+ 0.32；溴甲酚紫：ACA = 1.00（RID）+ 1.27。溴甲酚紫方法之间的相互相关性优于溴甲酚绿方法。冻干血清可用于白蛋白标准化。通过高效液相色谱（HPLC）对冻干血清和液体血清的白蛋白组分进行分析，未发现白蛋白聚合存在显著差异。纯化的白蛋白，无论聚合程度如何，在染料结合法中的反应都非常不同。溴甲酚紫方法显示出对纯化白蛋白的高估，溴甲酚绿方法则显示出低估。这些差异的原因尚不清楚。与溴甲酚绿相比，非人白蛋白与溴甲酚紫的反应较差。
