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用溴甲酚紫染料进行血清白蛋白测定。

Serum albumin assay with bromcresol purple dye.

作者信息

Parviainen M T, Harmoinen A, Jokela H

出版信息

Scand J Clin Lab Invest. 1985 Oct;45(6):561-4. doi: 10.3109/00365518509155259.

Abstract

Serum albumin was assayed with bromcresol purple using routine clinical chemical photometric analysers. Bromcresol purple and bromcresol green dyes were compared, and albumin assays using these dyes were compared to those made by radial immunodiffusion and rocket immunoelectrophoresis. Bromcresol purple seems to be insensitive to all serum proteins except albumin, and this dye is correlated linearly with radial immunodiffusion and with rocket electrophoresis.

摘要

使用常规临床化学光度分析仪,采用溴甲酚紫法测定血清白蛋白。对溴甲酚紫和溴甲酚绿染料进行了比较,并将使用这些染料的白蛋白测定法与放射免疫扩散法和火箭免疫电泳法进行了比较。溴甲酚紫似乎对除白蛋白以外的所有血清蛋白均不敏感,并且该染料与放射免疫扩散法和火箭电泳法呈线性相关。

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