Shibata Y, Arima T, Yamamoto T
J Microsc. 1984 Oct;136(Pt 1):121-3. doi: 10.1111/j.1365-2818.1984.tb02552.x.
We have developed a simple new method of rotary shadowing, double-axis rotary replication, for observing three-dimensional structures in deep-etched, rapid-frozen tissues. The technical details of this method are described and compared with the conventional fixed-angle rotary shadowing procedure.