Margaritis L H, Elgsaeter A, Branton D
J Cell Biol. 1977 Jan;72(1):47-56. doi: 10.1083/jcb.72.1.47.
Rotary replication has been adapted to freeze-etching and evaluated using T4 polyheads, erythrocyte ghosts, and chloroplast membranes. Conventional electron microscopy, electron diffraction, and optical diffraction and filtering indicate that platinum-carbon rotary replication renders radially symmetrical contrast and 25 A resolution to freeze-etched specimens so as to clarify subunit structure not normally evident in unidirectional shadow replicas.
旋转复制已应用于冷冻蚀刻,并使用T4多角体、红细胞血影和叶绿体膜进行了评估。传统电子显微镜、电子衍射以及光学衍射和滤波表明,铂 - 碳旋转复制能为冷冻蚀刻标本呈现径向对称的对比度和25埃的分辨率,从而清晰显示单向阴影复制品中通常不明显的亚基结构。