用于冷冻蚀刻的旋转复型法。
Rotary replication for freeze-etching.
作者信息
Margaritis L H, Elgsaeter A, Branton D
出版信息
J Cell Biol. 1977 Jan;72(1):47-56. doi: 10.1083/jcb.72.1.47.
Abstract
Rotary replication has been adapted to freeze-etching and evaluated using T4 polyheads, erythrocyte ghosts, and chloroplast membranes. Conventional electron microscopy, electron diffraction, and optical diffraction and filtering indicate that platinum-carbon rotary replication renders radially symmetrical contrast and 25 A resolution to freeze-etched specimens so as to clarify subunit structure not normally evident in unidirectional shadow replicas.
摘要
旋转复制已应用于冷冻蚀刻，并使用T4多角体、红细胞血影和叶绿体膜进行了评估。传统电子显微镜、电子衍射以及光学衍射和滤波表明，铂 - 碳旋转复制能为冷冻蚀刻标本呈现径向对称的对比度和25埃的分辨率，从而清晰显示单向阴影复制品中通常不明显的亚基结构。
相似文献
1
Rotary replication for freeze-etching.用于冷冻蚀刻的旋转复型法。
J Cell Biol. 1977 Jan;72(1):47-56. doi: 10.1083/jcb.72.1.47.
2
An ultrastructural study of the cytoplasmic aspects of erythrocyte membranes by a quick-freezing and deep-etching method.用快速冷冻和深度蚀刻法对红细胞膜细胞质方面进行的超微结构研究。
J Anat. 1992 Apr;180 ( Pt 2)(Pt 2):315-20.
3
A new freeze-etch unit for freeze-etch rotary shadowing, low temperature freeze-fracturing and conventional freeze-etching.一种用于冷冻蚀刻旋转阴影、低温冷冻断裂和传统冷冻蚀刻的新型冷冻蚀刻装置。
J Microsc. 1978 May;113(1):83-94. doi: 10.1111/j.1365-2818.1978.tb00097.x.
4
5
Membrane skeleton in fresh unfixed erythrocytes as revealed by a rapid-freezing and deep-etching method.用快速冷冻和深度蚀刻法揭示的新鲜未固定红细胞中的膜骨架。
J Anat. 1994 Oct;185 ( Pt 2)(Pt 2):415-20.
6
7
Freeze fracture and freeze etching.冷冻断裂和冷冻蚀刻。
Methods Mol Biol. 2014;1117:95-132. doi: 10.1007/978-1-62703-776-1_6.
8
9
An improved specimen table for the Balzers freeze etching system BAF 400.一种用于巴尔泽斯冷冻蚀刻系统BAF 400的改进型样品台。
J Microsc. 1982 Aug;127(Pt 2):227-31. doi: 10.1111/j.1365-2818.1982.tb00416.x.
10
Interpreting the results of freeze-etching.解读冷冻蚀刻的结果。
J Microsc. 1977 Sep;111(1):117-24. doi: 10.1111/j.1365-2818.1977.tb00051.x.
引用本文的文献
1
The origins and evolution of freeze-etch electron microscopy.冷冻蚀刻电子显微镜的起源与发展
J Electron Microsc (Tokyo). 2011;60 Suppl 1(Suppl 1):S3-29. doi: 10.1093/jmicro/dfr044.
2
Deep-etch EM reveals that the early poxvirus envelope is a single membrane bilayer stabilized by a geodetic "honeycomb" surface coat.深度蚀刻电子显微镜显示，早期痘病毒包膜是由大地测量学上的“蜂窝状”表面涂层稳定的单膜双层结构。
J Cell Biol. 2005 Apr 25;169(2):269-83. doi: 10.1083/jcb.200412169.
3
Hydrodynamic properties of human erythrocyte band 3 solubilized in reduced Triton X-100.溶解于还原型曲拉通X-100中的人红细胞带3的流体动力学性质。
Biophys J. 1999 Apr;76(4):2043-55. doi: 10.1016/S0006-3495(99)77361-3.
4
Gap junctions of the muscles of the small and large intestine.小肠和大肠肌肉的缝隙连接。
Cell Tissue Res. 1981;219(3):469-88. doi: 10.1007/BF00209987.
5
The formation of intravesicular calcium phosphate deposits in microsomes of smooth muscle. A comparison with sarcoplasmic reticulum of skeletal muscle.平滑肌微粒体中膀胱内磷酸钙沉积物的形成。与骨骼肌肌浆网的比较。
Histochemistry. 1981;70(2):139-50. doi: 10.1007/BF00493206.
6
Dimeric arrangement and structure of the membrane-bound acetylcholine receptor studied by electron microscopy.通过电子显微镜研究膜结合型乙酰胆碱受体的二聚体排列和结构。
EMBO J. 1982;1(5):541-7. doi: 10.1002/j.1460-2075.1982.tb01206.x.
7
Freeze-fracturing and deep-etching with the volatile cryoprotectant ethanol reveals true membrane surfaces of kidney structures.使用挥发性冷冻保护剂乙醇进行冷冻断裂和深度蚀刻可揭示肾脏结构的真实膜表面。
Cell Tissue Res. 1980;210(1):57-69. doi: 10.1007/BF00232141.
8
Protease effects on the structure of acetylcholine receptor membranes from Torpedo californica.蛋白酶对加州电鳐乙酰胆碱受体膜结构的影响。
J Cell Biol. 1980 Jun;85(3):823-38. doi: 10.1083/jcb.85.3.823.
9
The band 3-rich membrane of llama erythrocytes: studies on cell shape and the organization of membrane proteins.羊驼红细胞富含带3蛋白的膜：关于细胞形状和膜蛋白组织的研究
J Membr Biol. 1983;72(3):161-71. doi: 10.1007/BF01870583.
10
Ultrastructural aspects of rapid-frozen, deep-etched and rotary-shadowed synaptosomes.快速冷冻、深度蚀刻和旋转阴影突触体的超微结构方面
Cell Tissue Res. 1983;231(2):347-55. doi: 10.1007/BF00222186.
本文引用的文献
1
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.人红细胞无血红蛋白空泡的制备及其化学特性
Arch Biochem Biophys. 1963 Jan;100:119-30. doi: 10.1016/0003-9861(63)90042-0.
2
Structure of the tubular variants of the head of bacteriophage T4 (polyheads). I. Arrangement of subunits in some classes of polyheads.噬菌体T4头部管状变体（多面体头部）的结构。I. 某些类别的多面体头部中亚基的排列
J Mol Biol. 1972 Apr 14;65(3):469-88. doi: 10.1016/0022-2836(72)90202-1.
3
[Improved resolution in freeze-etching].[冷冻蚀刻中分辨率的提高]
Histochemie. 1969;20(2):133-42. doi: 10.1007/BF00268707.
4
Fracture faces in frozen outer segments from the guinea pig retina.豚鼠视网膜冷冻外节中的断裂面。
Z Zellforsch Mikrosk Anat. 1968;91(4):586-603. doi: 10.1007/BF00455276.
5
Application of the freeze-fracture technique to natural membranes.冷冻断裂技术在天然膜中的应用。
Methods Enzymol. 1974;32:35-44. doi: 10.1016/0076-6879(74)32006-x.
6
Maturation of the head of bacteriophage T4. IV. The proteins of the core of the tubular polyheads and in vitro cleavage of the head proteins.噬菌体T4头部的成熟。IV. 管状多面体头部核心的蛋白质及头部蛋白质的体外切割
Virology. 1974 Dec;62(2):483-99. doi: 10.1016/0042-6822(74)90409-7.
7
Recent progress in the freeze-etching technique.冷冻蚀刻技术的最新进展。
Philos Trans R Soc Lond B Biol Sci. 1971 May 27;261(837):121-31. doi: 10.1098/rstb.1971.0042.
Zotero 插件