通过液-凝胶色谱法和气-液色谱-质谱联用技术分析接受鹅去氧胆酸治疗的健康受试者粪便中类固醇的代谢谱。

Analysis of metabolic profiles of steroids in faeces of healthy subjects undergoing chenodeoxycholic acid treatment by liquid-gel chromatography and gas-liquid chromatography-mass spectrometry.

作者信息

Owen R W, Thompson M H, Hill M J

出版信息

J Steroid Biochem. 1984 Nov;21(5):593-600. doi: 10.1016/0022-4731(84)90336-4.

Abstract

The multicomponent analysis of faecal steroids is described. Steroids were removed from faeces by solvent stripping in a Soxhlet apparatus and the resulting extracts were fractionated by diethylaminohydroxypropyl Sephadex column chromatography into neutral sterols, free bile acids, glycine conjugated bile acids, taurine conjugated bile acids and sulphated steroids. In this study the method has been applied for faecal steroid analyses of healthy subjects undergoing chenodeoxycholic acid therapy. Chenodeoxycholic acid administration causes a considerable increase in the concentration of faecal lithocholic acid which is a known comutagenic bile acid. Furthermore it has been shown that conjugated bile acids can account for between 10 and 20% of the faecal bile acid pool. The method described is convenient and may be useful for epidemiological studies which require a large number of faecal samples to be analysed.

摘要

本文描述了粪便类固醇的多组分分析方法。通过索氏提取器用溶剂萃取法从粪便中去除类固醇，所得提取物通过二乙氨基羟丙基葡聚糖凝胶柱色谱法分离为中性固醇、游离胆汁酸、甘氨酸结合胆汁酸、牛磺酸结合胆汁酸和硫酸化类固醇。在本研究中，该方法已应用于接受鹅去氧胆酸治疗的健康受试者的粪便类固醇分析。给予鹅去氧胆酸会导致粪便中石胆酸浓度显著增加，而石胆酸是一种已知的共诱变胆汁酸。此外，研究表明结合胆汁酸可占粪便胆汁酸池的10%至20%。所描述的方法简便易行，可能对需要分析大量粪便样本的流行病学研究有用。