Orsi R, Brunelli G, Zorzi F
Microsurgery. 1984;5(4):191-6. doi: 10.1002/micr.1920050405.
Light microscope and ultrastructural changes in the rat kidney in tourniquet shock are described. The animals (24) were divided into two series; in the first one, the hind limb was kept at room temperature; in the second series, prerefrigeration was used. The effects of different ischemia were studied (2,4,8, and 12 hours for warm ischemia; 4,8,12, and 16 hours for cold ischemia). The animals were sacrificed at 3, 10, and 30 days. Pathological changes were evident, consisting in an increase of cytosomes in the proximal tubules and apical vacuolation in collecting ducts; numerous cytosomes including myelin-like structures were also seen. Large differences between warm and cold ischemia alterations were found. The changes in the tubules were clearly less important in cold ischemia. The authors conclude that there is a protective effect in regard to prevention of renal pathology affordable by prerefrigeration of a limb for reimplantation.
本文描述了大鼠在止血带休克时肾脏的光学显微镜和超微结构变化。将24只动物分为两组；第一组，后肢置于室温下；第二组，采用预冷藏。研究了不同缺血时间的影响（热缺血为2、4、8和12小时；冷缺血为4、8、12和16小时）。在3、10和30天时处死动物。病理变化明显，表现为近端小管中胞质体增多以及集合管顶端空泡化；还可见到包括髓鞘样结构在内的大量胞质体。发现热缺血和冷缺血改变之间存在很大差异。冷缺血时肾小管的变化明显较轻。作者得出结论，肢体预冷藏用于再植对预防肾脏病理改变具有保护作用。
