Hirst L W, Auer C, Cohn J, Tseng S C, Khodadoust A A
Ophthalmology. 1984 Oct;91(10):1147-53. doi: 10.1016/s0161-6420(84)34167-7.
Wide-field specular microscopy, slit-lamp examination, and pachometry were performed on 22 successful hard contact lens wearers and 22 controls matched for age, race, sex, and refractive error. A minimum of 600 cells per control and 1200 per contact lens wearer were manually digitized from the specular photomicrographs. Frequency distributions of cell areas were compared between the two groups using the parameters of mean, median, standard deviation, coefficient of variation, skewness and kurtosis. Slit-lamp examination was normal and there was no significant difference in corneal thickness between the patient group and the control group. Comparison of mean, median, and standard deviation also revealed no significant difference, but skewness (P less than .001), kurtosis (P less than .001) and coefficient of variation (P less than .004) were greater in the hard contact lens wear group. Hard contact lens wearing time correlated with increasing pleomorphism (P less than .05). Specular microscopy also revealed morphologic changes including deep stromal striae, intra- and extracellular "blackout" areas, and clustering of extremely small and large cells. The possible relationship between endothelial hypoxia and structural stromal changes are discussed.
对22名成功佩戴硬性隐形眼镜者和22名年龄、种族、性别及屈光不正相匹配的对照组进行了广域镜面显微镜检查、裂隙灯检查和角膜厚度测量。从镜面显微照片中手动数字化每个对照组至少600个细胞以及每个隐形眼镜佩戴者至少1200个细胞。使用均值、中位数、标准差、变异系数、偏度和峰度参数比较两组细胞面积的频率分布。裂隙灯检查正常，患者组与对照组之间角膜厚度无显著差异。均值、中位数和标准差的比较也未显示出显著差异，但硬性隐形眼镜佩戴组的偏度（P小于0.001）、峰度（P小于0.001）和变异系数（P小于0.004）更大。硬性隐形眼镜佩戴时间与多形性增加相关（P小于0.05）。镜面显微镜检查还发现了形态学变化，包括深层基质条纹、细胞内和细胞外“遮光”区域以及极小和极大细胞的聚集。讨论了内皮细胞缺氧与基质结构变化之间的可能关系。
