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[淋巴细胞间期死亡的分子机制。大鼠胸腺染色质辐射后降解产物中转录序列的分布]

[Molecular mechanisms of interphase death of lymphoid cells. Distribution of transcribable sequences in products of the postradiation degradation of rat thymus chromatin].

作者信息

Borisova E A, Zhivotovskiĭ B D, Khanson K P

出版信息

Radiobiologiia. 1984 Nov-Dec;24(6):723-7.

PMID:6515005
Abstract

The method of hybridization was used to study the presence of the transcribed sequences in PDN subfractions. As a component of mononucleosomes possessing a high electrophoretic motility DNA was enriched with the diverse unique transcribed siquences. It was shown that during the postirradiation degradation of the chromatin the mononucleosome fragments of PDN were mainly formed from the genome sites active in transcription whereas the high molecular weight fragments contained its repressed sequences.

摘要

采用杂交方法研究了PDN亚组分中转录序列的存在情况。作为具有高电泳迁移率的单核小体的一个组成部分,DNA富含各种独特的转录序列。结果表明,在染色质的辐射后降解过程中,PDN的单核小体片段主要由转录活跃的基因组位点形成,而高分子量片段则包含其受抑制的序列。

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