Adams J D, Lauterburg B H, Mitchell J R
Res Commun Chem Pathol Pharmacol. 1984 Dec;46(3):401-10.
The plasma concentrations of glutathione and glutathione disulfide were measured in rats following the administration of diquat, metronidazole, nitrofurantoin, doxorubicin, dimethylnitrosamine or carbon tetrachloride. None of these agents significantly influenced the plasma concentrations of glutathione. Diquat, metronidazole and nitrofurantoin acutely increased plasma glutathione disulfide by 4 to 13 fold from 0.3 +/- 0.1 microM to 3.8 +/- 1.1 microM, 2.0 +/- 0.5 microM and 1.2 +/- 0.3 microM, respectively. Carbon tetrachloride, doxorubicin and dimethylnitrosamine did not affect plasma glutathione disulfide. The determination of plasma glutathione disulfide identifies compounds that generate potentially toxic amounts of reactive oxygen species during their metabolism and helps to distinguish these compounds from xenobiotics which generate organic free radicals and electrophilic metabolites under toxicologically relevant conditions.
在给予大鼠敌草快、甲硝唑、呋喃妥因、阿霉素、二甲基亚硝胺或四氯化碳后，测定了大鼠血浆中谷胱甘肽和二硫化谷胱甘肽的浓度。这些药物均未显著影响血浆谷胱甘肽的浓度。敌草快、甲硝唑和呋喃妥因可使血浆二硫化谷胱甘肽急性升高4至13倍，分别从0.3±0.1微摩尔/升升至3.8±1.1微摩尔/升、2.0±0.5微摩尔/升和1.2±0.3微摩尔/升。四氯化碳、阿霉素和二甲基亚硝胺不影响血浆二硫化谷胱甘肽。血浆二硫化谷胱甘肽的测定可识别在代谢过程中产生潜在毒性量活性氧的化合物，并有助于将这些化合物与在毒理学相关条件下产生有机自由基和亲电代谢物的外源性物质区分开来。
