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使用氢质子自旋回波核磁共振（1H spin echo NMR）和高效液相色谱法（HPLC）来证实阿霉素诱导完整的HeLa细胞中谷胱甘肽的消耗。

The use of 1H spin echo NMR and HPLC to confirm doxorubicin induced depletion of glutathione in the intact HeLa cell.

作者信息

al-Kabban M, Watson I D, Stewart M J, Reglinski J, Smith W E, Suckling C J

机构信息

Department of Pathological Biochemistry, Glasgow Royal Infirmary, UK.

出版信息

Br J Cancer. 1988 Jun;57(6):553-8. doi: 10.1038/bjc.1988.127.

DOI:10.1038/bjc.1988.127
PMID:3408642
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2246460/
Abstract

The effects of doxorubicin on the cellular biochemistry of the HeLa cell using 1H spin echo nuclear magnetic resonance spectroscopy (NMR) of the intact and viable cell in conjunction with dual wavelength HPLC of cell lysates is reported. Directly dose-related changes were observed in lactate and reduced glutathione concentration. Doxorubicin induces a time-dependent depletion of the cytosolic pool of glutathione and a change in the glycolytic pattern of the cell. The glutathione depletion could be partially reversed by controlled pre-treatment of the cells with N-acetylcysteine and cysteine, the protection being linked to the intracellular concentration of the thiol.

摘要

报道了使用完整活细胞的1H自旋回波核磁共振光谱（NMR）结合细胞裂解物的双波长高效液相色谱法（HPLC）研究阿霉素对HeLa细胞细胞生物化学的影响。观察到乳酸和还原型谷胱甘肽浓度与剂量直接相关的变化。阿霉素诱导细胞溶质谷胱甘肽池随时间的消耗以及细胞糖酵解模式的改变。用N-乙酰半胱氨酸和半胱氨酸对细胞进行可控预处理可部分逆转谷胱甘肽的消耗，这种保护作用与细胞内硫醇的浓度有关。

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本文引用的文献

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Effect of manipulation on 32P loss from tissue culture cells.操作对组织培养细胞中³²P损失的影响。
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Estimation of glutathione in rat liver by reversed-phase high-performance liquid chromatography: separation from cysteine and gamma-glutamylcysteine.采用反相高效液相色谱法测定大鼠肝脏中的谷胱甘肽：与半胱氨酸和γ-谷氨酰半胱氨酸的分离
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L1210(A) mouse lymphoma cells depleted of glutathione with L-buthionine-S-R-sulfoximine proliferate in tissue culture.用L-丁硫氨酸-S-R-亚砜亚胺去除谷胱甘肽的L1210(A)小鼠淋巴瘤细胞在组织培养中增殖。
Biochem Biophys Res Commun. 1984 Sep 17;123(2):626-32. doi: 10.1016/0006-291x(84)90275-4.
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New aspects of glutathione biochemistry and transport: selective alteration of glutathione metabolism.谷胱甘肽生物化学与转运的新进展：谷胱甘肽代谢的选择性改变
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Glutathione.谷胱甘肽
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N.m.r. studies of red cells.红细胞的核磁共振研究。
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