Al-Fares A M, Mira S A, el-Sayed Y M
Ther Drug Monit. 1984;6(4):454-7. doi: 10.1097/00007691-198412000-00013.
Digoxin in serum was measured by fluorescence polarization immunoassay (FPIA) and by radioimmunoassay (RIA). Intraday precision of the FPIA method determined by replicate analysis of three controls (low, medium, and high) gave coefficients of variation of 5.41, 2.67, and 2.37%, respectively. The interday coefficients of variation were 5.71, 4.55, and 1.93% for the low, medium, and high controls, respectively. The mean recovery for three spiked controls was 98%. Quantitative results obtained by FPIA on serum samples from patients receiving digoxin were compared with the results obtained by RIA. The correlation coefficient was 0.989. The stability of the standard curve in FPIA was evaluated by recalibrating the instrument at two intervals, 21 days apart, and superimposing the standard curve. No significant changes were found in polarization values.
采用荧光偏振免疫分析法（FPIA）和放射免疫分析法（RIA）测定血清中的地高辛。通过对三个对照品（低、中、高浓度）进行重复分析确定的FPIA方法日内精密度，其变异系数分别为5.41%、2.67%和2.37%。低、中、高对照品的日间变异系数分别为5.71%、4.55%和1.93%。三个加标对照品的平均回收率为98%。将FPIA法对接受地高辛治疗患者的血清样本获得的定量结果与RIA法获得的结果进行比较。相关系数为0.989。通过在间隔21天的两个时间点重新校准仪器并叠加标准曲线，评估了FPIA中标准曲线的稳定性。未发现偏振值有显著变化。
