Camsonne R, Moulin M A, Albessard F, Perault M
Ther Drug Monit. 1984;6(4):471-3. doi: 10.1097/00007691-198412000-00017.
A simple and rapid reversed-phase C-18 high-performance liquid chromatography (HPLC) assay without a prior extraction step for dihydroquinidine (HQD) in serum is described. The method is selective and useful for monitoring HQD and its presumed metabolites, quinidine (QD), N-oxide QD, 3-hydroxy QD (3-OHQD), and 10-11-diol QD. The detection limit for HQD was 0.2 +/- 0.1 mg/L, and the peak heights--concentration curve was linear between 0.5 and 10.0 mg/L. The coefficients of variation within and between runs were identical, 5.0 +/- 1.5%. A 100 +/- 2.5% recovery was obtained by direct injection into the chromatograph of the supernatant following acetonitrile protein precipitation. The total assay time was less than 15 min.
本文描述了一种简单快速的反相C-18高效液相色谱（HPLC）分析法，用于血清中二氢奎尼丁（HQD）的检测，无需事先进行萃取步骤。该方法具有选择性，可用于监测HQD及其推测的代谢产物奎尼丁（QD）、N-氧化物QD、3-羟基QD（3-OHQD）和10-11-二醇QD。HQD的检测限为0.2±0.1mg/L，峰高-浓度曲线在0.5至10.0mg/L之间呈线性。批内和批间变异系数相同，为5.0±1.5%。通过对乙腈沉淀蛋白后的上清液直接进样到色谱仪中，回收率为100±2.5%。总分析时间少于15分钟。
