Rakhit A, Kunitani M, Holford N H, Riegelman S
Clin Chem. 1982 Jul;28(7):1505-9.
We describe a simple, rapid, and specific assay for quinidine and its known metabolites in plasma, urine, and bile. Plasma proteins are precipitated by adding acetonitrile, which also contains the internal standard. The supernatant fluid is evaporated and the reconstituted residue is separated on a reversed-phase column, with fluorescence detection. The standard curve is linear and results are reproducible over the clinical concentration ranges: quinidine 0.4 to 8.0 mg/L and the three metabolites (quinidine 10,11-diol, 3-hydroxyquinidine, and quinidine-N-oxide) 0.05 to 1.5 mg/L. As little as 10 micrograms of the N-oxide metabolite per liter and 1 microgram of the other analytes per liter can be quantitated in 0.5 mL of plasma, urine, or bile. With the use of an automated chromatographic system, many samples can be analyzed in a continuous run.
我们描述了一种用于检测血浆、尿液和胆汁中奎尼丁及其已知代谢物的简单、快速且特异的分析方法。通过加入含有内标的乙腈使血浆蛋白沉淀。将上清液蒸发，复溶后的残渣在反相柱上分离，采用荧光检测。标准曲线呈线性，在临床浓度范围内结果可重现：奎尼丁为0.4至8.0 mg/L，三种代谢物（奎尼丁10,11 -二醇、3 -羟基奎尼丁和奎尼丁 - N -氧化物）为0.05至1.5 mg/L。在0.5 mL血浆、尿液或胆汁中，每升低至10微克的N -氧化物代谢物和每升1微克的其他分析物均可被定量。使用自动色谱系统，可连续分析许多样本。
