Goldberg I, Rock J S, Ben-Bassat A, Mateles R I
Biotechnol Bioeng. 1976 Dec;18(12):1657-68. doi: 10.1002/bit.260181202.
Several bacteria utilizing C1-compounds as sole carbon sources were grown on these substrates in continuous culture. The molar yield values (g of cell dry wt/mol of substrate utilized) of bacteria which utilize C1-compounds via the ribulose monophosphate pathway were between 15.7 to 17.3 when grown on methanol; while the molar yield values of bacteria which use the serine pathway for the assimilation of C1-compounds varied between 9.8 and 13.1. The molar yield values of different bacteria which use the serine pathway decreased as the oxidation levels of the C1-growth substrates increased. On formaldehyde the values were between 7.2 to 9.6, whereas on formate the values varied from 3.3 to 6.9. It appears that bacteria utilize C1-compounds more efficiently via the ribulose monophosphate pathway than via the serine pathway. The oxidation step from methanol to formaldehyde (and from methylamine to formaldehyde) in the bacteria studied may be energy yielding. A comparison has been made between the experimental yield values obtained and theoretical values.
几种以C1化合物作为唯一碳源的细菌在连续培养中利用这些底物生长。通过磷酸戊糖途径利用C1化合物的细菌，在以甲醇为底物生长时，其摩尔产率值（每利用1摩尔底物产生的细胞干重克数）在15.7至17.3之间；而通过丝氨酸途径同化C1化合物的细菌，其摩尔产率值在9.8至13.1之间。随着C1生长底物氧化水平的增加，使用丝氨酸途径的不同细菌的摩尔产率值降低。在甲醛上，该值在7.2至9.6之间，而在甲酸上，该值在3.3至6.9之间变化。看来细菌通过磷酸戊糖途径比通过丝氨酸途径更有效地利用C1化合物。在所研究的细菌中，从甲醇到甲醛（以及从甲胺到甲醛）的氧化步骤可能产生能量。已将获得的实验产率值与理论值进行了比较。
