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[恶臭假单胞菌质粒DNA的特征]

[Characteristics of Pseudomonas putida plasmid DNAs].

作者信息

Agabalian A S, Zakharian R A, Akopian S M, Bakunts K A, Israelian Iu A

出版信息

Mikrobiologiia. 1978 Jan-Feb;47(1):97-100.

PMID:651695
Abstract

Physico-chemical characteristics of plasmid DNAs isoalted from Pseudomonas putida G7 were studied as well as the behavior of these DNAs in th eourse of chromatography on columns with Sepharose 4B and kieselguhr with methylated albumin (MAC). This strain was found to contain several plasmid DNAs having molecular weights of 33-36X10(6), 15-18X10(6), and 3-5X10(6) dalton. The plasmid DNAs of biodegradation are supposed to be located in the vicinity of chromosomes, and only a small part of them is characterized by extrachromosomal localization.

摘要

对从恶臭假单胞菌G7中分离出的质粒DNA的物理化学特性进行了研究,并研究了这些DNA在琼脂糖4B柱和涂有甲基化白蛋白的硅藻土(MAC)柱上的色谱行为。发现该菌株含有几种分子量分别为33 - 36×10⁶、15 - 18×10⁶和3 - 5×10⁶道尔顿的质粒DNA。具有生物降解功能的质粒DNA被认为位于染色体附近,并且只有一小部分具有染色体外定位的特征。

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