• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

从恶臭假单胞菌中分离质粒脱氧核糖核酸。

Isolation of plasmid deoxyribonucleic acid from Pseudomonas putida.

作者信息

Palchaudhuri S, Chakrabarty A

出版信息

J Bacteriol. 1976 Apr;126(1):410-6. doi: 10.1128/jb.126.1.410-416.1976.

DOI:10.1128/jb.126.1.410-416.1976
PMID:816778
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC233297/
Abstract

Conditions suitable for reproducible recovery of covalently closed circular deoxyribonucleic acid from strains of Pseudomonas putida containing degradative plasmids (CAM, SAL, OCT, etc.) have been defined. These degradative plasmids could not be isolated by the usual procedure, whereas RP1, an R factor of the P group, present in the isogenic strain of P. putida, was isolated equally well by either the usual procedure or the modified procedure. Characterization by electron microscopy of RP1 deoxyribonucleic acid confirmed the molecular weight (about 40 X 10(6)) previously determined by sucrose gradient centrifugation.

摘要

已确定了适合从含有降解质粒（CAM、SAL、OCT等）的恶臭假单胞菌菌株中可重复回收共价闭合环状脱氧核糖核酸的条件。这些降解质粒不能通过常规方法分离，而恶臭假单胞菌同基因菌株中存在的P组R因子RP1，通过常规方法或改良方法都能同样良好地分离出来。通过电子显微镜对RP1脱氧核糖核酸进行表征，证实了先前通过蔗糖梯度离心法测定的分子量（约40×10⁶）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea6/233297/400e3dbd7d8b/jbacter00317-0427-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea6/233297/f4d79c77acd4/jbacter00317-0426-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea6/233297/400e3dbd7d8b/jbacter00317-0427-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea6/233297/f4d79c77acd4/jbacter00317-0426-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea6/233297/400e3dbd7d8b/jbacter00317-0427-a.jpg

相似文献

1
Isolation of plasmid deoxyribonucleic acid from Pseudomonas putida.从恶臭假单胞菌中分离质粒脱氧核糖核酸。
J Bacteriol. 1976 Apr;126(1):410-6. doi: 10.1128/jb.126.1.410-416.1976.
2
Detection and characterization of plasmids in Pseudomonas glycinea.大豆假单胞菌中质粒的检测与鉴定
J Bacteriol. 1977 Jul;131(1):224-8. doi: 10.1128/jb.131.1.224-228.1977.
3
Transposition of a beta-lactamase locus from RP1 into Pseudomonas putida degradative plasmids.β-内酰胺酶基因座从RP1转位至恶臭假单胞菌降解性质粒中。
J Bacteriol. 1977 Feb;129(2):809-14. doi: 10.1128/jb.129.2.809-814.1977.
4
Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation.通过染料浮力密度离心法从细菌裂解物中获得更准确的共价闭合环状质粒与染色体比率的方法。
J Bacteriol. 1977 Apr;130(1):148-53. doi: 10.1128/jb.130.1.148-153.1977.
5
Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2).从天蓝色链霉菌A3(2)中分离共价闭合环状脱氧核糖核酸。
J Bacteriol. 1975 Feb;121(2):416-21. doi: 10.1128/jb.121.2.416-421.1975.
6
Isolation of plasmid deoxyribonucleic acid from two strains of Bacteroides.从两株拟杆菌中分离质粒脱氧核糖核酸。
J Bacteriol. 1975 Oct;124(1):503-10. doi: 10.1128/jb.124.1.503-510.1975.
7
[Plasmid characteristics of naphthalene and salicylate biodegradation in Pseudomonas putida].
Mikrobiologiia. 1980 Nov-Dec;49(6):931-5.
8
Characterization of plasmid deoxyribonucleic acid from Neisseria gonorrhoeae.淋病奈瑟菌质粒脱氧核糖核酸的特性分析。
Infect Immun. 1974 Oct;10(4):712-7. doi: 10.1128/iai.10.4.712-717.1974.
9
Isolation of covalently closed circular deoxyribonucleic acid from bacteria which produce exocellular nuclease.从产生胞外核酸酶的细菌中分离共价闭合环状脱氧核糖核酸。
J Bacteriol. 1973 Jan;113(1):508-9. doi: 10.1128/jb.113.1.508-509.1973.
10
Molecular sizes and relationships of TOL plasmids in Pseudomonas.假单胞菌中TOL质粒的分子大小及相互关系
J Bacteriol. 1977 Jun;130(3):1274-80. doi: 10.1128/jb.130.3.1274-1280.1977.

引用本文的文献

1
Indicator technique for antimetabolic toxin production by phytopathogenic species of pseudomonas.指示技术用于检测植物病原菌假单胞菌产生的抗代谢毒素。
Appl Environ Microbiol. 1980 Jan;39(1):25-9. doi: 10.1128/aem.39.1.25-29.1980.
2
Plasmid control of 6-aminohexanoic acid cyclic dimer degradation enzymes of Flavobacterium sp. KI72.黄杆菌属KI72菌株6-氨基己酸环状二聚体降解酶的质粒控制
J Bacteriol. 1980 Jul;143(1):238-45. doi: 10.1128/jb.143.1.238-245.1980.
3
In vivo genesis of a transposon carrying the histidine genes of Escherichia coli K-12.

本文引用的文献

1
Control by endogenously synthesized arginine of the formation of ornithine transcarbamylase in Escherichia coli.大肠杆菌中内源性合成的精氨酸对鸟氨酸转氨甲酰酶形成的调控。
J Bacteriol. 1961 Feb;81(2):236-40. doi: 10.1128/jb.81.2.236-240.1961.
2
A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.一种用于检测和分离闭环双链DNA的染料浮力密度法：HeLa细胞中的闭环DNA
Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21. doi: 10.1073/pnas.57.5.1514.
3
Compatibility between two F' factors in an Escherichia coli strain bearing a chromosomal mutation affecting DNA synthesis.
携带大肠杆菌K-12组氨酸基因的转座子在体内的产生。
Mol Gen Genet. 1984;193(1):172-8. doi: 10.1007/BF00327433.
4
Naphthalene plasmids in pseudomonads.假单胞菌中的萘质粒。
J Bacteriol. 1982 Mar;149(3):1096-101. doi: 10.1128/jb.149.3.1096-1101.1982.
5
Role of nucleases in the isolation of plasmid deoxyribonucleic acid from Pseudomonas cepacia 4G9.核酸酶在从洋葱伯克霍尔德菌4G9中分离质粒脱氧核糖核酸中的作用。
J Bacteriol. 1980 Aug;143(2):1057-9. doi: 10.1128/jb.143.2.1057-1059.1980.
6
Structure of a naturally occurring plasmid with genes for enterotoxin production and drug resistance.一种具有产肠毒素基因和耐药性基因的天然存在质粒的结构。
J Bacteriol. 1981 Jan;145(1):97-105. doi: 10.1128/jb.145.1.97-105.1981.
7
Use of a known plasmid and transposon as tracers for the incompatibility classification of a cryptic plasmid.使用已知质粒和转座子作为隐蔽质粒不相容性分类的示踪剂。
Mol Gen Genet. 1982;186(2):282-8. doi: 10.1007/BF00331863.
8
Characterization of the OCT plasmid encoding alkane oxidation and mercury resistance in Pseudomonas putida.恶臭假单胞菌中编码烷烃氧化和汞抗性的OCT质粒的特性分析
J Bacteriol. 1986 Feb;165(2):650-3. doi: 10.1128/jb.165.2.650-653.1986.
9
Isolation and characterization of plasmid from the Bacillus brevis var. G.-B. cells.
Mol Gen Genet. 1978 Aug 17;164(2):195-204. doi: 10.1007/BF00267384.
10
Isolation and characterization of the pesticide-degrading plasmid pJP1 from Alcaligenes paradoxus.从嗜碱假单胞菌中分离并鉴定农药降解质粒pJP1
J Bacteriol. 1978 Sep;135(3):798-804. doi: 10.1128/jb.135.3.798-804.1978.
在携带影响DNA合成的染色体突变的大肠杆菌菌株中两个F'因子之间的相容性。
J Mol Biol. 1971 Apr 28;57(2):319-33. doi: 10.1016/0022-2836(71)90349-4.
4
Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli.细菌中的非染色体抗生素抗性。II. 从奇异变形杆菌和大肠杆菌中分离出的R因子的分子性质。
J Mol Biol. 1970 Jun 28;50(3):671-87. doi: 10.1016/0022-2836(70)90092-6.
5
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.大肠杆菌中的超螺旋环状DNA-蛋白质复合物：纯化及诱导转化为开放环状DNA形式
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66. doi: 10.1073/pnas.62.4.1159.
6
Detection and characterization of plasmids in Pseudomonas aeruginosa strain PAO.铜绿假单胞菌PAO菌株中质粒的检测与鉴定
J Bacteriol. 1973 Apr;114(1):424-33. doi: 10.1128/jb.114.1.424-433.1973.
7
Genetic basis of the biodegradation of salicylate in Pseudomonas.假单胞菌中水杨酸盐生物降解的遗传基础。
J Bacteriol. 1972 Nov;112(2):815-23. doi: 10.1128/jb.112.2.815-823.1972.
8
General method for the isolation of plasmid deoxyribonucleic acid.质粒脱氧核糖核酸的分离通用方法。
J Bacteriol. 1973 Nov;116(2):1064-6. doi: 10.1128/jb.116.2.1064-1066.1973.
9
Characterization by electron microscopy of fused F-prime factors in Escherichia coli.大肠杆菌中融合F-prime因子的电子显微镜表征
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1873-6. doi: 10.1073/pnas.69.7.1873.
10
Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid.恶臭假单胞菌（arvilla）mt-2对苯甲酸酯和甲基苯甲酸酯的代谢：TOL质粒存在的证据
J Bacteriol. 1974 Oct;120(1):416-23. doi: 10.1128/jb.120.1.416-423.1974.