LaManna J C, McCracken K A
Anal Biochem. 1984 Oct;142(1):117-25. doi: 10.1016/0003-2697(84)90525-6.
Intracellular pH in the intact, normally perfused rat brain cortex was determined by rapid scanning reflectance spectrophotometry of Neutral Red. Neutral Red, a pH indicator dye, was administered intraperitoneally to rats. Reflectance spectra recorded from the exposed dural surface of 11 anesthetized rats were used to calculate an intracellular pH of 7.04 +/- 0.01. Detailed studies on the interactions of the dye with brain tissue were carried out in vitro to define the in vivo calibration curves. In addition, the physiological effect of dye administration on systemic blood pressure was determined, as well as uptake curves for Neutral Red into plasma and brain. It is concluded that Neutral Red can be used as an in vivo brain intracellular pH indicator and compares favorably with other methods of brain intracellular pH measurement with respect to accuracy, sensitivity, noninvasiveness, and stability and has the potential to exceed any existing method in time resolution.
通过对中性红进行快速扫描反射分光光度法测定完整的、正常灌注的大鼠大脑皮层中的细胞内pH值。中性红是一种pH指示染料，通过腹腔注射给予大鼠。从11只麻醉大鼠暴露的硬脑膜表面记录的反射光谱用于计算细胞内pH值为7.04±0.01。在体外对染料与脑组织的相互作用进行了详细研究，以确定体内校准曲线。此外，还测定了染料给药对全身血压的生理影响，以及中性红进入血浆和大脑的摄取曲线。得出的结论是，中性红可用作体内脑内细胞内pH指示剂，在准确性、灵敏度、非侵入性和稳定性方面与其他脑内细胞内pH测量方法相比具有优势，并且在时间分辨率方面有可能超过任何现有方法。
