Unni E, Kesari K V, Rao M R
Biochem Biophys Res Commun. 1984 Dec 14;125(2):454-62. doi: 10.1016/0006-291x(84)90562-x.
The seminiferous tubules of rat testes contain a protease and heat sensitive factor capable of stimulating (3H)-thymidine incorporation into quiescent cultures of NIH 3T3 mouse fibroblast cells. This mitogenic factor activity was only 4% in the testes of vitamin A deficient-retinoic acid maintained rats as compared to that of normal rats. Supplementation of retinyl acetate to these vitamin A deficient rats for 4 days resulted in a 56% recovery in the mitogenic factor activity while by day 16, the recovery was 80 percent. Incorporation of (3H)-thymidine into DNA of the seminiferous tubules of vitamin A deficient rats was only 46% of that of normal rats which was restored to normal levels by retinyl acetate supplementation for 24 days.
大鼠睾丸的生精小管含有一种蛋白酶和热敏感因子，该因子能够刺激（3H）-胸腺嘧啶核苷掺入NIH 3T3小鼠成纤维细胞的静止培养物中。与正常大鼠相比，在缺乏维生素A但用视黄酸维持的大鼠睾丸中，这种促有丝分裂因子活性仅为4%。给这些维生素A缺乏的大鼠补充醋酸视黄酯4天，促有丝分裂因子活性恢复了56%，而到第16天，恢复率为80%。维生素A缺乏大鼠生精小管DNA中（3H）-胸腺嘧啶核苷的掺入量仅为正常大鼠的46%，通过补充醋酸视黄酯24天可恢复到正常水平。
