Schuette W H, Shackney S E, Plowman F A, Tipton H W, Smith C A, MacCollum M A
Cytometry. 1984 Nov;5(6):652-6. doi: 10.1002/cyto.990050616.
A multibeam optical detection system has been developed with a high optical efficiency, achieved through a reduction in the number of optical interfaces employed in the system. This reduction is made possible by a combination of employing simple lenses, gluing the objective lens directly upon the face of the flow cuvette and the extraction of only one fluorescence signal from each laser beam. A modified flow chamber is also described that includes fluidic resistance elements for the elimination of most of the electric shielding normally associated with electronic cell volume measurements.
