de Josselin de Jong J E, Hartwijk P, Verkerk T, Jongkind J F
Cytometry. 1984 Nov;5(6):657-9. doi: 10.1002/cyto.990050617.
The two laser beams in a dual-laser fluorescence-activated cell sorter FACS-II can be aligned and focused independently on the sample stream with an additional unit, which can be fitted easily on the optical bench of the FACS. The unit consists of two spherical lenses, which have been mounted in separate holders and can be moved in three directions by way of micrometer gauges. The lenses, which have different focal lengths, have been cut off on one side so each laser beam only passes one lens. The setup has been tested using the flow analysis of a suspension of double-stained chicken red blood cells. The histograms of both fluorescence signals showed normal distributions with a coefficient of variation of approximately 6%. After willful interference with the adjustments, the laser beams could be readily readjusted within five minutes.
在双激光荧光激活细胞分选仪FACS-II中，两束激光可通过一个附加装置独立对准并聚焦到样本流上，该装置可轻松安装在FACS的光学工作台上。该装置由两个球面透镜组成，它们安装在单独的支架中，并可通过微米计在三个方向上移动。这两个焦距不同的透镜一侧被截断，因此每束激光仅通过一个透镜。已使用双染色鸡红细胞悬液的流动分析对该装置进行了测试。两种荧光信号的直方图均显示出正态分布，变异系数约为6%。在故意干扰调整后，激光束可在五分钟内轻松重新调整。
