McAthey P, Kilbey B
Mutat Res. 1978 May;50(2):175-80. doi: 10.1016/0027-5107(78)90022-2.
In agreement with the results obtained in Escherichia coli by other workers and our own previous data, the kinetics with which spontaneous mutations to resistance to the 12,13-epoxytrichothecene trichodermin accumulate in a lysine auxotroph of Schizosaccharomyces pombe are dependent upon the nutrilite used to limit the growth of the population. Under conditions of glucose-limitation mutation accumulation is proportional to generation time, while under lysine-limitation it becomes proportional to chronological time. In contrast to observations made in bacterial system, however, no significant change in the DNA content per cell is noted in slow growing cultures grown under amino acid starvation. These findings help to eliminate some of the theories put forward to explain the differential mutational responses observed under different growth limiting regimes.
与其他研究者在大肠杆菌中获得的结果以及我们自己先前的数据一致，在粟酒裂殖酵母的赖氨酸营养缺陷型中，对12,13 - 环氧单端孢霉烯族毒素木霉菌素产生抗性的自发突变积累动力学取决于用于限制群体生长的营养素。在葡萄糖限制条件下，突变积累与世代时间成正比，而在赖氨酸限制条件下，它与时间成正比。然而，与在细菌系统中的观察结果相反，在氨基酸饥饿条件下生长缓慢的培养物中，未观察到每个细胞的DNA含量有显著变化。这些发现有助于排除一些为解释在不同生长限制条件下观察到的差异突变反应而提出的理论。
