Purser C, Baltar A, Ho I K, Hume A S
J Chromatogr. 1984 Nov 9;311(1):135-40. doi: 10.1016/s0378-4347(00)84699-x.
A method for the extraction and quantification of cefoxitin in blood and bone samples is described in this paper. The procedure, which prepares biological material for reversed-phase high-performance liquid chromatographic analysis is convenient, rapid and reproducible. It also allows for use of cephalothin as an internal standard in measuring serum cefoxitin levels. Conventional extraction procedures, involving use of organic solvents, generally yield drug recoveries of 60-80%. Use of Baker--10 SPE disposable extraction columns allowed us to consistently obtain greater than 98% recovery of both cefoxitin and cephalothin. Methods for quantification of the extracted drugs include comparison of peak ratios (for serum) or peak heights (for bone) to first-order equations obtained from regression analyses.
本文介绍了一种血液和骨样本中头孢西丁的提取及定量方法。该方法为反相高效液相色谱分析制备生物材料，操作方便、快速且具有可重复性。在测定血清头孢西丁水平时，它还允许使用头孢噻吩作为内标。涉及使用有机溶剂的传统提取方法，药物回收率通常为60 - 80%。使用Baker - 10 SPE一次性提取柱，使我们能够始终如一地获得高于98%的头孢西丁和头孢噻吩回收率。提取药物的定量方法包括将峰面积比（用于血清）或峰高（用于骨）与通过回归分析得到的一阶方程进行比较。
