Cox S K, Burnette J D, Huss B T, Frazier D
Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Knoxville 37901, USA.
J Chromatogr B Biomed Sci Appl. 1998 Jan 23;705(1):145-8. doi: 10.1016/s0378-4347(97)00486-6.
A simple, rapid and sensitive method for the clean-up and analysis of cefoxitin in serum and tissue is described. Serum (0.5 ml) and tissue (100 mg) samples after homogenization underwent high speed centrifugation. Chromatography was performed on a muBondapak C18 cartridge using a mobile phase of 0.005 M potassium dihydrogen phosphate-acetonitrile-glacial acetic acid (77.5:22:0.5, v/v/v) with a flow-rate of 2.0 ml/min. Ultraviolet detection occurred at 235 nm. The procedure produced a linear curve for the concentration range 100-5000 ng/ml. The assay produced accurate, repeatable and rapid results for both tissue and serum samples without the need for chemical extraction.
本文描述了一种用于血清和组织中头孢西丁净化与分析的简单、快速且灵敏的方法。血清（0.5 ml）和组织（100 mg）匀浆后的样品进行高速离心。使用0.005 M磷酸二氢钾-乙腈-冰醋酸（77.5:22:0.5，v/v/v）作为流动相，流速为2.0 ml/min，在μBondapak C18柱上进行色谱分析。在235 nm处进行紫外检测。该方法在100 - 5000 ng/ml的浓度范围内产生线性曲线。该测定法对组织和血清样品均能产生准确、可重复且快速的结果，无需化学提取。
