Maier C E, Singer M
J Comp Neurol. 1984 Dec 10;230(3):459-64. doi: 10.1002/cne.902300313.
When a newt limb is amputated and begins to regrow, regenerating axons exert a neurotrophic influence on the limb regeneration process. Previous studies have shown that direct manipulation of limb nerves, by electrical stimulation or a conditioning lesion, elevates protein synthesis, increases neurotization and accelerates growth of the limb bud. Since exogenously supplied gangliosides accelerate axonal sprouting in regenerating nerves, we wanted to know whether gangliosides would similarly affect limb regeneration. To test this, regrowing limb buds were either infused with or immersed into gangliosides, or animals were injected intraperitoneally with gangliosides. Infused gangliosides elevated protein synthesis in limb buds 15% and increased the number of axons in limb buds 45% by 6 hours after infusion. Regenerating limb bud morphogenesis was initiated 3-4 days earlier in animals receiving i.p. injections of gangliosides every 12 hours. Similarly, limbs immersed daily in gangliosides began regrowth sooner than contralateral controls and this advantage was maintained throughout the period of observation. These findings indicate that treatment with gangliosides has a salutary effect on limb regeneration.
当蝾螈肢体被截断并开始再生时，再生的轴突会对肢体再生过程施加神经营养影响。先前的研究表明，通过电刺激或预处理损伤直接操控肢体神经，可提高蛋白质合成、增强神经支配并加速肢芽生长。由于外源性供应的神经节苷脂可加速再生神经中的轴突发芽，我们想知道神经节苷脂是否会同样影响肢体再生。为了对此进行测试，将再生的肢芽注入或浸入神经节苷脂中，或者给动物腹腔注射神经节苷脂。注入神经节苷脂后6小时，肢芽中的蛋白质合成提高了15%，肢芽中的轴突数量增加了45%。在每12小时接受一次腹腔注射神经节苷脂的动物中，再生肢芽的形态发生提前3 - 4天开始。同样，每天浸入神经节苷脂中的肢体比对侧对照肢体更早开始再生，并且在整个观察期内都保持了这一优势。这些发现表明，用神经节苷脂治疗对肢体再生具有有益作用。
