Moţa G, Dobre M A
J Immunol Methods. 1984 Dec 31;75(2):247-55. doi: 10.1016/0022-1759(84)90108-x.
A method for the determination and removal of circulating immune complexes in pathological sera was developed using human secretory or dimeric myeloma IgA covalently bound to Sepharose 4B. IgA-Sepharose 4B was able to selectively bind heat or antigen-aggregated human IgG (circulating immune complexes) but not monomeric IgG. The absorbent was also able to remove a very high proportion (95%) of circulating immune complexes from pathological sera as determined by a turbidimetric technique. An immunoradiometric assay for the direct measurement of circulating immune complexes is described. The assay uses IgA-Sepharose 4B as an absorbent (for the binding of IgG immune complexes from sera) and 125I-rabbit anti-IgG antibody (for the quantitation of IgG immune complexes bound to IgA-Sepharose 4B). The mean value obtained for pathological sera (27.1 +/- 0.9) was significantly higher than that of normal sera (4.8 +/- 0.5).
利用与人分泌型或二聚体骨髓瘤IgA共价结合的琼脂糖4B，开发了一种测定和去除病理性血清中循环免疫复合物的方法。IgA-琼脂糖4B能够选择性结合热或抗原聚集的人IgG（循环免疫复合物），但不能结合单体IgG。通过比浊技术测定，该吸附剂还能够从病理性血清中去除很高比例（95%）的循环免疫复合物。本文描述了一种直接测定循环免疫复合物的免疫放射分析方法。该分析使用IgA-琼脂糖4B作为吸附剂（用于结合血清中的IgG免疫复合物）和125I-兔抗IgG抗体（用于定量结合到IgA-琼脂糖4B上的IgG免疫复合物）。病理性血清的平均值（27.1±0.9）显著高于正常血清（4.8±0.5）。
