Levinson S S, Goldman J
Clin Chem. 1983 Dec;29(12):2035-9.
We describe absorbance nephelometry of immunoglobulin G (IgG) in immune complexes (IC) with use of anti-IgG after precipitating the IgG from serum by three treatments with polyethylene glycol, 25 g/L final concentration. The three-step procedure removed all of the monomeric IgG, eliminating interference with the IC assay. Analytical recovery of IC was nearly 100%. Both the standard curve and results for control sera showed good day-to-day reproducibility, with CVs of less than 8%. Eighteen of 20 patients with diseases involving IC showed distinctly increased concentrations of IC; no increases were seen in 15 normal persons. Measurement of IC in 44 samples by this assay and a C1q assay gave a Spearman rank correlation coefficient of 0.76. The assay appears suited to routine use with manual double-beam spectrophotometers and stable nephelometers. The procedure is inexpensive, straightforward, and easy to perform.
我们描述了在通过终浓度为25 g/L的聚乙二醇进行三次处理从血清中沉淀出免疫球蛋白G（IgG）后，使用抗IgG对免疫复合物（IC）中的IgG进行吸光度散射比浊法检测。该三步法去除了所有单体IgG，消除了对IC检测的干扰。IC的分析回收率接近100%。标准曲线和对照血清的结果均显示出良好的日间重复性，变异系数小于8%。20例患有涉及IC疾病的患者中有18例IC浓度明显升高；15名正常人未见升高。用该检测方法和C1q检测方法对44份样本进行IC检测，Spearman等级相关系数为0.76。该检测方法似乎适用于使用手动双光束分光光度计和稳定的散射比浊仪进行常规检测。该方法成本低廉、操作简单、易于实施。
