Bergevin G H, Red T, Bonakdar M, Pope S, Wells F, Wright J R
Physiol Chem Phys Med NMR. 1984;16(4):307-16.
Copper cluster ions which contain 14 copper atoms and 12 penicillamine ligands produce no indications of toxicity when injected intravenously in doses of 12 mg/kg (as the hydrated sodium salt). These ions pass freely through the Bowman's capsules and are eliminated with the urinary effluent. The blood plasma and blood serum kinetics of the process may be followed with selectivity and sensitivity using copper's flame atomic absorbance at 324.7 nm, and a first order rate law with a half life of approximately ten minutes is observed. Clearance obtained by this method is inulin-like. The copper cluster ion does not readily permeate the erythrocyte membrane, and its distribution immediately following intravenous injection appears to be essentially that of the plasma volume. Since serum or plasma preparation for atomic absorbance measurements is minimal, the method might have applications in rapid determinations of renal clearance.
含有14个铜原子和12个青霉胺配体的铜簇离子，以12毫克/千克（作为水合钠盐）的剂量静脉注射时，未显示出毒性迹象。这些离子可自由通过鲍曼氏囊，并随尿液排出。利用铜在324.7纳米处的火焰原子吸收，可以选择性和灵敏地追踪该过程的血浆和血清动力学，并且观察到符合一级速率定律，半衰期约为十分钟。通过这种方法获得的清除率类似于菊粉。铜簇离子不易渗透红细胞膜，静脉注射后其分布似乎基本上与血浆量相同。由于用于原子吸收测量的血清或血浆制备量极少，该方法可能在快速测定肾清除率方面有应用。
