Bourget C, Flood C, Longcope C
Steroids. 1984 Feb;43(2):225-33. doi: 10.1016/0039-128x(84)90041-2.
Male rabbits were infused at a constant rate with 3H-androstenedione/14C-estrone (n = 5) or 3H-testosterone/14C-estradiol-17 beta (n = 3) for 3 1/2 hr and blood samples were obtained over the last hour and analyzed for radioactivity as androstenedione (A), testosterone (T), estrone (E1), estradiol-17 beta (E2 beta) and estradiol-17 alpha (E2 alpha). The mean value for the metabolic clearance rate of androstenedione (MCRA) was 85 +/- 10 l/day/kg, which was significantly greater than the mean MCRE1 59 +/- 10 l/day/kg. MCRT, 42 +/- 8 l/day/kg, and MCRE2 beta, 45 +/- 9 l/day/kg were not different. The conversion ratio of androstenedione to testosterone (CRA,T) was greater than CRT,A but for the estrogens, CRE2 beta, E1 was greater than CRE1,E2 beta. CRE2 beta, E2 alpha was greater than CRE1,E2 alpha. The overall aromatization of androstenedione to estrone, the fraction of 3H-androstenedione infused into the blood and measured as 3H-estrone in blood [( rho]A,E1BB) was 0.0005 +/- 0.0001 and for [rho]T,E2 beta BB was 0.0012 +/- 0.0006. In the rabbit both sex hormone binding globulin (SHBG) and albumin binding may effect the MCRs, and peripheral aromatization of androgens occurs to a far lesser degree than in humans and primates.
给雄性兔子以恒定速率输注3H-雄烯二酮/14C-雌酮（n = 5）或3H-睾酮/14C-雌二醇-17β（n = 3），持续3.5小时，在最后一小时采集血样，分析其中作为雄烯二酮（A）、睾酮（T）、雌酮（E1）、雌二醇-17β（E2β）和雌二醇-17α（E2α）的放射性。雄烯二酮代谢清除率（MCRA）的平均值为85±10升/天/千克，显著高于雌酮代谢清除率（MCRE1）的平均值59±10升/天/千克。睾酮代谢清除率（MCRT）为42±8升/天/千克，雌二醇-17β代谢清除率（MCRE2β）为45±9升/天/千克，二者无差异。雄烯二酮向睾酮的转化比（CRA,T）大于睾酮向雄烯二酮的转化比（CRT,A），但对于雌激素，雌二醇-17β向雌酮的转化率（CRE2β,E1）大于雌酮向雌二醇-17β的转化率（CRE1,E2β）。雌二醇-17β向雌二醇-17α的转化率（CRE2β,E2α）大于雌酮向雌二醇-17α的转化率（CRE1,E2α）。雄烯二酮向雌酮的总体芳香化作用，即输注到血液中的3H-雄烯二酮中以血液中3H-雌酮形式测得的比例[（ρ）A,E1BB]为0.0005±0.0001，而[（ρ）T,E2βBB]为0.0012±0.0006。在兔子中，性激素结合球蛋白（SHBG）和白蛋白结合都可能影响代谢清除率，雄激素的外周芳香化程度远低于人类和灵长类动物。
