Hulsebosch C E, Coggeshall R E, Perez-Polo J R
Brain Res. 1984 Dec 3;323(1):1-10. doi: 10.1016/0006-8993(84)90259-2.
Axons were counted in rat dorsal roots three segments cranial and three segments caudal to a spinal hemisection done at birth. The animals were placed in three groups: (1) hemisected (HEMI), (2) hemisected and given nerve growth factor (NGF) daily (HEMI + NGF) and (3) hemisected and given antibodies to NGF daily (HEMI + anti-NGF). They were sacrificed at 1 month. In the HEMI and HEMI + NGF groups, there were 20% and 13% more unmyelinated axons, respectively, in roots of the operated side. We interpret these differences to be the result of sprouting of unmyelinated axons as a result of denervation, and exogenous NGF does not seem to affect the response. By contrast, the unmyelinated axon responses in HEMI + anti-NGF animals are different. These animals have approximately 7% more unmyelinated axons on the denervated side and 50% more axons on the normal side than in the other animals we studied. The results on the anti-NGF animals are interpreted as indicating sprouting in response to a chemical denervation caused by NGF removal and the response is more dramatic on the unoperated side.
对出生时进行脊髓半横切的大鼠，计数其脊髓半横切部位头侧三个节段和尾侧三个节段的背根中的轴突。将动物分为三组：(1) 半横切组 (HEMI)，(2) 半横切且每日给予神经生长因子 (NGF) 组 (HEMI + NGF)，(3) 半横切且每日给予NGF抗体组 (HEMI + anti-NGF)。在1个月时将它们处死。在HEMI组和HEMI + NGF组中，手术侧神经根中无髓鞘轴突分别多20%和13%。我们将这些差异解释为去神经支配导致无髓鞘轴突发芽的结果，外源性NGF似乎不影响这种反应。相比之下，HEMI + anti-NGF动物的无髓鞘轴突反应不同。与我们研究的其他动物相比，这些动物去神经侧的无髓鞘轴突大约多7%，正常侧的轴突多50%。抗NGF动物的结果被解释为表明对因去除NGF引起的化学去神经支配的反应性发芽，且在未手术侧反应更明显。
