Sado Y, Okigaki T, Takamiya H, Seno S
J Clin Lab Immunol. 1984 Dec;15(4):199-204.
Experimental autoimmune glomerulonephritis with pulmonary hemorrhage was induced in rats by a single injection of soluble antigen obtained from bovine glomerular basement membrane. Groups of rats were injected with various amounts of the nephritogenic antigen with Freund's complete adjuvant and killed 10 weeks later. Glomerulonephritis with linear deposition of IgG along the glomerular basement membrane developed in 7 of 10 rats injected with 10 micrograms of the antigen and all 20 rats of other 2 groups with 100 or 1,000 micrograms of the antigen. Pulmonary hemorrhage was observed in 21 of 27 rats with nephritis, and it was particularly severe in those injected with 1,000 micrograms. Glomerulonephritis with pulmonary hemorrhage and the linear deposition of IgG along with the glomerular basement membrane suggests that this model resembles human anti-glomerular basement membrane antibody-induced glomerulonephritis including Goodpasture's syndrome. The nephritic rats showed symptoms of nephrotic syndrome such as hypoalbuminemia, hypercholesterolemia and hypertriglyceremia, and the severities of these symptoms clearly depended on the degree of proteinuria.
通过单次注射从牛肾小球基底膜获得的可溶性抗原，在大鼠中诱发伴有肺出血的实验性自身免疫性肾小球肾炎。给几组大鼠注射不同剂量的致肾炎抗原与弗氏完全佐剂，10周后处死。在注射10微克抗原的10只大鼠中有7只以及其他两组分别注射100或1000微克抗原的所有20只大鼠中，均出现了沿肾小球基底膜有IgG线性沉积的肾小球肾炎。在27只患肾炎的大鼠中有21只观察到肺出血，在注射1000微克抗原的大鼠中尤为严重。伴有肺出血以及沿肾小球基底膜有IgG线性沉积的肾小球肾炎表明，该模型类似于人类抗肾小球基底膜抗体诱发的肾小球肾炎，包括古德帕斯彻综合征。患肾炎的大鼠出现了肾病综合征的症状，如低白蛋白血症、高胆固醇血症和高甘油三酯血症，这些症状的严重程度明显取决于蛋白尿的程度。
