Sado Y, Naito I, Akita M, Okigaki T
J Clin Lab Immunol. 1986 Apr;19(4):193-9.
Experimental autoimmune glomerulonephritis with pulmonary hemorrhage was induced in rats of 4 inbred strains, F344/DuCrj, LEW/Crj, WKY/NCrj and SHR/NCrj, by the injection of a nephritogenic antigen from bovine glomerular basement membrane. The severity and clinical course of glomerulonephritis within the strains were very similar, but those between the strains were different. Among them, F344 rats demonstrated mild glomerulonephritis with petechial pulmonary hemorrhage. LEW rats showed more severe symptoms from pulmonary hemorrhage than F344 rats and had heavy, long-lasting proteinuria that caused nephrotic syndrome. WKY rats showed very severe glomerulonephritis with pulmonary hemorrhage, and had azotemia by 4 weeks after the injection. SHR rats had both severe glomerulonephritis and severe pulmonary hemorrhage. Investigation of the dose-effect relationship of the nephritogenic antigen in F344 and WKY rats revealed that the minimum dose required to induce nephritis in F344 and WKY rats was 10 micrograms and 3 micrograms respectively, and that the injection of more than a certain amount (i.e., 30 micrograms in F344, 10 micrograms in WKY) of the antigenic material induced almost the same severity of disease. These results indicate that genetic differences in experimental animals influence the severity of renal damage, suggesting that the use of inbred strains is necessary for adequate study of experimental glomerulonephritis.
通过注射来自牛肾小球基底膜的致肾炎抗原，在4个近交系大鼠（F344/DuCrj、LEW/Crj、WKY/NCrj和SHR/NCrj）中诱导出伴有肺出血的实验性自身免疫性肾小球肾炎。各品系内肾小球肾炎的严重程度和临床病程非常相似，但品系间则有所不同。其中，F344大鼠表现为轻度肾小球肾炎伴有瘀点性肺出血。LEW大鼠肺出血症状比F344大鼠更严重，且有大量、持续时间长的蛋白尿，导致肾病综合征。WKY大鼠表现出非常严重的伴有肺出血的肾小球肾炎，注射后4周出现氮质血症。SHR大鼠既有严重的肾小球肾炎又有严重的肺出血。对F344和WKY大鼠致肾炎抗原的剂量效应关系进行研究发现，诱导F344和WKY大鼠发生肾炎所需的最小剂量分别为10微克和3微克，且注射超过一定量（即F344为30微克，WKY为10微克）的抗原物质会诱发几乎相同严重程度的疾病。这些结果表明实验动物的遗传差异会影响肾损伤的严重程度，提示在充分研究实验性肾小球肾炎时使用近交系是必要的。
