Sado Y, Naito I
Division of Immunology Research, Shigei Medical Research Institute, Okayama, Japan.
Br J Exp Pathol. 1987 Oct;68(5):695-704.
Experimental autoimmune glomerulonephritis was induced in inbred WKY/NCrj rats and Wistar (closed colony) rats by a single injection of isologous or homologous soluble antigens from glomerular and tubular basement membranes. Glomerular and tubular basement membranes were trypsin digested and applied to an affinity column to which rabbit antibodies to bovine nephritogenic antigen had been coupled. The adsorbed fraction was nephritogenic when it was injected into rat footpads with Freund's complete adjuvant. Glomerulonephritis with long-lasting proteinuria and haematuria developed 2 to 3 weeks after the injection, and it was characterized histologically by endocapillary hypercellularity of mononuclear cells, capsular adhesion, sclerosis of capillary tufts, and crescent formation. Immunofluorescence study revealed the linear deposition of rat IgG along the glomerular basement membrane. Some rats with the nephritis had pulmonary hemorrhage. These results suggest that this experimental model is similar to the experimental glomerulonephritis induced in rats by bovine nephritogenic antigen, and to human anti-glomerular basement membrane antibody-induced glomerulonephritis including Goodpasture's syndrome.
通过单次注射来自肾小球和肾小管基底膜的同种或同源可溶性抗原，在近交系WKY/NCrj大鼠和Wistar（封闭群体）大鼠中诱导实验性自身免疫性肾小球肾炎。将肾小球和肾小管基底膜用胰蛋白酶消化后应用于亲和柱，该亲和柱已偶联了针对牛致肾炎抗原的兔抗体。当将吸附部分与弗氏完全佐剂一起注射到大鼠足垫中时，它具有致肾炎性。注射后2至3周出现伴有持续性蛋白尿和血尿的肾小球肾炎，其组织学特征为单核细胞的毛细血管内细胞增多、包膜粘连、毛细血管襻硬化和新月体形成。免疫荧光研究显示大鼠IgG沿肾小球基底膜呈线性沉积。一些患有肾炎的大鼠出现肺出血。这些结果表明，该实验模型类似于牛致肾炎抗原诱导的大鼠实验性肾小球肾炎，以及人类抗肾小球基底膜抗体诱导的肾小球肾炎，包括古德帕斯丘综合征。
