Misra M, Egerton R F
Ultramicroscopy. 1984;15(4):337-43. doi: 10.1016/0304-3991(84)90128-1.
Electron radiation damage is one of the most severe problems in high resolution electron microscopy by biomolecules. The techniques of electron diffraction and electron energy-loss spectroscopy were applied to gain a better understanding of radiation damage in amino acids and nucleic acid bases. The results when compared with G-values for the release of ammonia and hydrogen sulphide from amino acids seem to indicate that bond scission is an important cause of radiation damage at moderate doses of irradiation. High resolution structural disorder in nucleic acid bases was found to involve loss of atoms peripheral to the main ring structure.
电子辐射损伤是生物分子高分辨率电子显微镜中最严重的问题之一。应用电子衍射和电子能量损失谱技术，以更好地了解氨基酸和核酸碱基中的辐射损伤。与氨基酸释放氨和硫化氢的G值相比，结果似乎表明，在中等辐照剂量下，键断裂是辐射损伤的一个重要原因。发现核酸碱基中的高分辨率结构紊乱涉及主环结构外围原子的损失。
