Humphrey B A, Marshall K C
Arch Microbiol. 1984 Dec;140(2-3):166-70. doi: 10.1007/BF00454920.
The marine Vibrio DW1 exhibited a positive response in heat output to a dialysis membrane surface in the presence of substrate (100 mM sodium glutamate) and, more particularly, in the absence of exogenous substrate (starvation conditions). The latter result paralleled the previously reported decrease in cell volume and increase in oxygen consumption by starving bacteria at a similar surface. Modified Morita's salts (MMS) did not extract nutrients from the dialysis membrane, but an artificial seawater containing tris buffer (ASW-tris) did extract surface active and nutrient materials from the membrane. The ASW-tris membrane extract and a commercial surfactant, Tween 85, were found to mimic the effects of the dialysis membrane surface by inducing a decrease in cell volume, and an increasing oxygen consumption and heat output of Vibrio DW1 even in the bulk liquid. The significance of the adsorption of naturally occurring surfactants at surfaces in relation to the behaviour of bacteria at the surfaces is discussed.
海洋弧菌DW1在存在底物（100 mM谷氨酸钠）时，对透析膜表面的热输出呈现阳性反应，更特别的是，在没有外源底物（饥饿条件）时也是如此。后一结果与之前报道的饥饿细菌在类似表面上细胞体积减小和耗氧量增加的情况相似。改良的森田盐（MMS）不会从透析膜中提取营养物质，但含有三羟甲基氨基甲烷缓冲液的人工海水（ASW-tris）会从膜中提取表面活性物质和营养物质。发现ASW-tris膜提取物和一种商业表面活性剂吐温85，即使在散装液体中，也能通过诱导弧菌DW1的细胞体积减小、耗氧量增加和热输出增加，来模拟透析膜表面的作用。讨论了天然表面活性剂在表面的吸附与细菌在表面行为的关系。
