Rosenstiel School of Marine and Atmospheric Science, Division of Marine and Atmospheric Chemistry, Miami, Florida 33149; and University of Miami, Department of Microbiology, Miami, Florida 33101.
Appl Environ Microbiol. 1983 Feb;45(2):411-7. doi: 10.1128/aem.45.2.411-417.1983.
Measurements were made of adsorption of a periphytic marine bacterium, glucose, and glutamic acid to inorganic particles in seawater and defined bacterial growth medium. Measurements of the metabolism of bacteria were made in the presence and absence of particles by microcalorimetry and radiorespirometry. It was found that hydroxyapatite adsorbs glutamic acid, but not glucose, from the experimental medium. It was also found that hydroxyapatite adsorbs essentially all of the bacteria from the medium when the bacterial concentration is approximately 6 x 10 bacteria per ml. If the bacterial concentration is approximately 6 x 10, then only a small fraction of cells become attached. It was therefore possible to select bacterial concentrations and organic nutrients so that bacterial attachment, organic nutrient adsorption, or both would occur in different experiments. In this experimental system the metabolism by attached and nonattached bacteria of adsorbing and nonadsorbing organic nutrients was measured. The results show that bacterial activity in this model system was not enhanced by the particles, regardless of whether the bacteria, the organic nutrient, or both were associated with the surface. In fact, the respiratory activity of the attached bacteria was diminished in comparison with that of free bacteria.
对附着于海洋微生物、葡萄糖和谷氨酸在海水中和特定细菌生长介质中的无机颗粒的吸附作用进行了测量。通过微量热法和放射性呼吸测量法，在有颗粒和无颗粒存在的情况下，对细菌的代谢进行了测量。研究发现，羟磷灰石可以从实验介质中吸附谷氨酸，但不能吸附葡萄糖。还发现，当细菌浓度约为 6 x 10 个细菌/ml 时，羟磷灰石几乎可以从培养基中吸附所有的细菌。如果细菌浓度约为 6 x 10 ，则只有一小部分细胞附着。因此，可以选择细菌浓度和有机养分，以便在不同的实验中发生细菌附着、有机养分吸附或两者兼而有之。在这个实验系统中，测量了附着和非附着细菌对吸附和非吸附有机养分的代谢作用。结果表明，无论细菌、有机养分还是两者都与表面有关，颗粒对附着细菌的代谢没有增强作用。事实上，与游离细菌相比，附着细菌的呼吸活性降低了。
