Seto-Ohshima A, Kitajima S, Sano M, Kato K, Mizutani A
Cell Struct Funct. 1984 Dec;9(4):337-44. doi: 10.1247/csf.9.337.
Calmodulin is a small, acidic, calcium-binding protein thought to regulate many cellular functions. In the brain of the adult mouse, calmodulin was found immunohistochemically to localize mainly in the neurons. In the developing brain, the immunoreactivity to anti-calmodulin antibody appeared early in the cells in the low brain stem but late in the cerebral cortex, hippocampus, and cerebellum, except for the deep cerebellar nuclei. The cells in the major proliferative layer present during early development, such as the matrix cells in the cerebral cortex and the cells in the external granular layer in the cerebellum, did not show the immunoreactivity. In the cerebral cortex, the migrating cells and the cells in the cortical plate were also negative while the deep cortical cells, which had probably settled in their final position, became positive. The comparison of these results with the immunohistochemical appearance of neuron specific enolase, a characteristic protein in the brain, suggested that calmodulin appeared with some maturation of the neurons as neuron specific enolase.
钙调蛋白是一种小的酸性钙结合蛋白，被认为可调节多种细胞功能。在成年小鼠大脑中，通过免疫组织化学方法发现钙调蛋白主要定位于神经元中。在发育中的大脑中，抗钙调蛋白抗体的免疫反应性在低位脑干的细胞中出现较早，但在大脑皮质、海马体和小脑中出现较晚，小脑深部核团除外。在早期发育过程中存在的主要增殖层中的细胞，如大脑皮质中的基质细胞和小脑中的外颗粒层中的细胞，未显示出免疫反应性。在大脑皮质中，迁移细胞和皮质板中的细胞也呈阴性，而可能已定居于最终位置的深部皮质细胞则呈阳性。将这些结果与神经元特异性烯醇化酶（一种大脑中的特征性蛋白）的免疫组织化学表现进行比较，表明钙调蛋白与神经元特异性烯醇化酶一样，随着神经元的一些成熟而出现。
