Seto-Ohshima A, Yamazaki Y, Kawamura N, Kitajima S, Sano M, Mizutani A
Histochemistry. 1987;86(4):337-43. doi: 10.1007/BF00494990.
Calmodulin (CaM) is a major calcium-binding protein in the brain, where its immunoreactivity is mainly localized in the neurons. In this study, ontogenical changes in the distribution of CaM in the nervous system of mouse embryos were investigated immunohistochemically using a specific antibody against CaM and an indirect immunoenzyme method. Immunoreactive staining was first observed in the marginal layer of the cranial neural tube after 9.5 days of gestation; thereafter, the amount of stained structures increased rapidly. Particularly intense staining was observed in the long neuronal processes extending from or into the brain and spinal cord primordia. Intense immunostaining was also observed in the optic nerve layer of early retinae from 12.5 days of gestation. The appearance of CaM immunoreactivity is thus an early event during neuronal differentiation, apparently concomitant with the initiation of axon extension and the appearance of neurofilament proteins.
钙调蛋白（CaM）是大脑中一种主要的钙结合蛋白，其免疫反应性主要定位于神经元中。在本研究中，使用抗CaM的特异性抗体和间接免疫酶法，通过免疫组织化学方法研究了小鼠胚胎神经系统中CaM分布的个体发生变化。妊娠9.5天后，在颅神经管的边缘层首次观察到免疫反应性染色；此后，染色结构的数量迅速增加。在从脑和脊髓原基延伸出或延伸至其中的长神经突起中观察到特别强烈的染色。从妊娠12.5天开始，在早期视网膜的视神经层中也观察到强烈的免疫染色。因此，CaM免疫反应性的出现是神经元分化过程中的一个早期事件，显然与轴突延伸的开始和神经丝蛋白的出现同时发生。
