Shiokawa K, Saito A, Kageura H, Higuchi K, Koga K, Yamana K
Cell Struct Funct. 1984 Dec;9(4):369-80. doi: 10.1247/csf.9.369.
Dorsal, ventral, animal and vegetal half-embryos of Xenopus laevis were isolated at the 8-cell stage, and their synthesis of protein examined during 12 h of culture. The kinetics of the uptake and incorporation of [35S]-methionine did not differ greatly among the four kinds of half-embryos. Two-dimensional gel electrophoreses showed that there were no detectable differences in the pattern of protein synthesis for the four types of half-embryos, at least during the first 6 h of culture which covered the period from the cleavage to late blastula stage. The patterns, however, changed greatly, especially between those of the animal and vegetal half-embryos, during the latter 6 h of culture which covered the period from the early gastrula to early neurula stage. These results are evidence that equal qualitative and quantitative maternal mRNA-dependent protein synthesis takes place in the four types of embryonic regions, including the vegetal half-embryos, although later in the post-blastular stages, syntheses of region-specific proteins start that are dependent on new genome expression.
在8细胞期分离非洲爪蟾的背侧、腹侧、动物极和植物极半胚胎，并在培养12小时期间检测它们的蛋白质合成情况。[35S] - 甲硫氨酸的摄取和掺入动力学在这四种半胚胎之间没有太大差异。二维凝胶电泳显示，至少在从卵裂到囊胚晚期的培养的前6小时内，这四种半胚胎的蛋白质合成模式没有可检测到的差异。然而，在培养的后6小时内，即从原肠胚早期到神经胚早期的阶段，这些模式发生了很大变化，尤其是动物极和植物极半胚胎之间。这些结果证明，在包括植物极半胚胎在内的四种胚胎区域中发生了等量的定性和定量的母体mRNA依赖性蛋白质合成，尽管在囊胚后期，开始合成依赖于新基因组表达的区域特异性蛋白质。
