Smith R C, Knowland J
Dev Biol. 1984 Jun;103(2):355-68. doi: 10.1016/0012-1606(84)90324-5.
Two-dimensional gel electrophoresis has been used to analyze protein synthesis in dorsal and ventral regions in embryonic stages of Xenopus laevis. Proteins specific either to dorsal or to ventral regions are synthesized for the first time at gastrulation, concomitant with morphological differentiation. The reliability of these proteins as markers of dorsal and ventral differentiation was tested by examining their synthesis in Uv-irradiated embryos, which have severely reduced capacity for dorsal development, reflected in reduced levels of the neuromuscular-specific enzyme acetylcholinesterase, but which continue to synthesize the great majority of proteins at normal rates. Synthesis of dorsal indicator proteins should be reduced or absent in these embryos, whereas ventral indicators should be synthesized at least to the same extent as in control embryos. Some of the putative dorsal and ventral indicators failed this test, but the majority were confirmed as reliable markers of dorsal and ventral differentiation, thus providing a connection between morphology and gene expression in the establishment of the dorsal-ventral axis in X. laevis.
二维凝胶电泳已被用于分析非洲爪蟾胚胎期背侧和腹侧区域的蛋白质合成。在原肠胚形成时首次合成了背侧或腹侧区域特有的蛋白质，这与形态分化同时发生。通过检测这些蛋白质在紫外线照射胚胎中的合成情况，来测试它们作为背侧和腹侧分化标志物的可靠性。紫外线照射胚胎的背侧发育能力严重降低，这反映在神经肌肉特异性酶乙酰胆碱酯酶水平降低，但它们仍以正常速率合成绝大多数蛋白质。在这些胚胎中，背侧指示蛋白的合成应该减少或缺失，而腹侧指示蛋白的合成至少应与对照胚胎中的合成程度相同。一些假定的背侧和腹侧指示蛋白未通过此测试，但大多数被确认为背侧和腹侧分化的可靠标志物，从而在非洲爪蟾背腹轴的建立过程中提供了形态与基因表达之间的联系。
