Miyata S, Kageura H, Kihara H K
Cell Differ. 1987 Jun;21(1):47-52. doi: 10.1016/0045-6039(87)90447-7.
Regional differences of proteins were studied by two-dimensional gel electrophoresis in early embryos of Xenopus laevis. Pairs of blastomeres on the dorso-ventral axis were isolated from 16- and 32-cell embryos. Some dorso-ventral differences have been detected at 32-cell embryos. The proteins which were clearly detectable in the vegetal cells of the ventral marginal zone were only faintly detectable or undetectable in those of the dorsal marginal zone, and a regionally specific spot was detected in dorsal blastomeres.
通过二维凝胶电泳研究了非洲爪蟾早期胚胎中蛋白质的区域差异。从16细胞期和32细胞期胚胎中分离出背腹轴上的卵裂球对。在32细胞期胚胎中检测到了一些背腹差异。在腹侧边缘区植物细胞中清晰可检测到的蛋白质,在背侧边缘区植物细胞中仅微弱可检测到或无法检测到,并且在背侧卵裂球中检测到一个区域特异性斑点。