Bland R E, Harrison C, Martin L E, Oxford J, Tanner R J
Int J Environ Anal Chem. 1984;18(1-2):25-35. doi: 10.1080/03067318408076989.
An automated continuous flow liquid-liquid extraction procedure is described for the separation of the H2-antagonist loxtidine from plasma samples containing two metabolites which interfere in the radioimmunoassay of the drug. The extraction of the bronchodilator salbutamol was studied using the DuPont Prep I automated liquid solid extraction apparatus, with a 12 cartridge capacity, and a vacuum extraction box designed in this laboratory to hold 30 Sep-pak C-18 (Waters Associates) cartridges. Twenty-four plasma samples per hour can be automatically processed with the Prep I. Although the vacuum box is not fully automated 45 plasma samples per hour can be processed. The Prep I can only be used with DuPont XAD, strong cation and anion exchange cartridges. Cartridges containing alumina, silica, florisil, cation and anion exchange resins and reverse phase packings can all be used with the vacuum extraction box. The latter costs only a fraction of the Prep I and therefore each analyst can have his own unit.
本文描述了一种自动化连续流动液-液萃取方法，用于从含有两种干扰该药物放射免疫测定的代谢物的血浆样品中分离H2拮抗剂洛替丁。使用具有12个柱管容量的杜邦Prep I自动液-固萃取装置以及本实验室设计的可容纳30个Sep-pak C-18（沃特世公司）柱管的真空萃取箱，研究了支气管扩张剂沙丁胺醇的萃取。使用Prep I每小时可自动处理24个血浆样品。虽然真空箱不是完全自动化的，但每小时可处理45个血浆样品。Prep I只能与杜邦XAD、强阳离子和阴离子交换柱管一起使用。含有氧化铝、硅胶、弗罗里硅土、阳离子和阴离子交换树脂以及反相填料的柱管均可与真空萃取箱一起使用。后者的成本仅为Prep I的一小部分，因此每个分析人员都可以拥有自己的装置。
