McCarthy P T, Atwal S, Sykes A P, Ayres J G
National Poisons Unit, Guys and Lewisham NHS Trust, London, UK.
Biomed Chromatogr. 1993 Jan-Feb;7(1):25-8. doi: 10.1002/bmc.1130070107.
A method is described for the determination of terbutaline and salbutamol in plasma from patients given maximal therapy for brittle asthma. The analytes were isolated by solid phase extraction on alkali-treated Bond-Elut, unmodified, silica columns and measured by high performance liquid chromatography with fluorescence detection (excitation wavelength 200 nm). The limits of detection for a 1 mL sample containing salbutamol and terbutaline were 1 microgram/L and 2.5 micrograms/L, respectively. The intra-assay precision (CV) for samples containing 25 micrograms/L was 3.6 and 5.0% respectively. This method was applied to the measurement of terbutaline in samples from patients given continuous infusions of the drug to assess whether this treatment might result in toxicity.
本文描述了一种用于测定接受脆性哮喘最大剂量治疗患者血浆中特布他林和沙丁胺醇的方法。通过在碱处理的Bond-Elut未改性硅胶柱上进行固相萃取分离分析物，并采用荧光检测（激发波长200nm）的高效液相色谱法进行测定。含沙丁胺醇和特布他林的1mL样品的检测限分别为1μg/L和2.5μg/L。含25μg/L样品的批内精密度（CV）分别为3.6%和5.0%。该方法应用于测定接受该药物持续输注患者的样品中的特布他林，以评估这种治疗是否可能导致毒性。
