Tsuchihashi H, Yadomae T, Miyazaki T
J Biochem. 1984 Dec;96(6):1789-97. doi: 10.1093/oxfordjournals.jbchem.a135012.
The occurrence of three forms, I, II, and III, of exo-beta-D-glucuronidase of the fungus Kobayasia nipponica was demonstrated. These three forms were purified 1,905-fold, 857-fold, and 357-fold, respectively. Forms I, II, and III of exo-beta-D-glucuronidase behaved differently on heparin-Sepharose chromatography, and differed in optimum pH (3.5, 3.2, and 2.6, respectively), pH-stability, Km (0.22, 0.16, and 0.13 mM, respectively), and Vmax (V) values. Their molecular weights, as estimated by gel filtration through Sephacryl S-200, were all 70,000; polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate gave a value of 72,000. These three forms were very active towards 1-4 linked beta-D-glucuronans, (Formula: see text) and (Formula: see text), but weak or inactive towards protuberic acid and several glycosaminoglycans.
已证实日本小笠原菌的外-β-D-葡萄糖醛酸酶存在I、II和III三种形式。这三种形式分别被纯化了1905倍、857倍和357倍。外-β-D-葡萄糖醛酸酶的I、II和III形式在肝素-琼脂糖层析上表现不同，在最适pH（分别为3.5、3.2和2.6）、pH稳定性、Km（分别为0.22、0.16和0.13 mM）和Vmax（V）值方面也存在差异。通过Sephacryl S-200凝胶过滤估计，它们的分子量均为70,000；在十二烷基硫酸钠存在下进行的聚丙烯酰胺凝胶电泳给出的值为72,000。这三种形式对1-4连接的β-D-葡糖醛聚糖（化学式：见原文）和（化学式：见原文）具有很高的活性，但对原丁酸和几种糖胺聚糖的活性较弱或无活性。
