日本小滨麦中的突起酸水解酶及其水解产物的表征
Protuberic acid-hydrolytic enzyme in Kobayasia nipponica and characterization of the hydrolytic products.
作者信息
Tsuchihashi H, Yadomae T, Miyazaki T
出版信息
J Biochem. 1983 Oct;94(4):1037-44. doi: 10.1093/oxfordjournals.jbchem.a134445.
When the cold water-extract of Kobayasia nipponica containing a glycuronan, protuberic acid (PA), was allowed to stand at room temperature, PA was hydrolyzed. The optimum conditions for this PA-hydrolysis were 37 degrees C and pH 4-5 in 0.1 M acetate buffer. Characterization of the hydrolytic products was performed by chemical analysis, and by 1H- and 13C-NMR spectroscopy. They were identified as D-GlcUA and O-(alpha-L-IdUAp)-(1-4)-D-GlcUA. These results suggest that PA-hydrolytic enzyme(s) include at least endo-beta-D-glucuronidase.
当含有葡糖醛酸聚糖、原块菌酸（PA）的日本块菌冷水提取物在室温下放置时，PA会发生水解。这种PA水解的最佳条件是在0.1M乙酸盐缓冲液中，温度为37℃，pH值为4 - 5。通过化学分析以及1H-和13C-核磁共振光谱对水解产物进行了表征。它们被鉴定为D-葡萄糖醛酸（D-GlcUA）和O-(α-L-艾杜糖醛酸)-(1→4)-D-葡萄糖醛酸（O-(alpha-L-IdUAp)-(1-4)-D-GlcUA）。这些结果表明，PA水解酶至少包括内切β-D-葡糖醛酸酶。
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