Miyazaki T, Yadomae T, Terui T, Yamada H, Kikuchi T
Biochim Biophys Acta. 1975 Apr 7;385(2):345-53. doi: 10.1016/0304-4165(75)90363-3.
A water-soluble glucuronan "protuberic acid", [alpha] 22-D minus 83.6 degrees, was isolated and purified from Kobayashia Nipponica, and its physicochemical properties were investigated. The purified protuberic acid was homogeneous as shown by zone electrophoresis, gel filtration over Sepharose 4B, and ultracentrifugation. The sedimentation coefficient was 1.8 S and its intrinsic viscosity was 1.1 dl/g. By gel filtration the molecular weight was estimated to be about 170 000. The results of periodate oxidation, methylation analysis, and partial acid hydrolysis indicated that this acidic polysaccharide has a linear structure of mainly 1, 4-linkages and containing an acid-labile linkage. Reduced protuberic acid, [alpha] 22-D minus 44 degrees, is also described.
从日本小林藻中分离并纯化出一种水溶性葡糖醛酸聚糖“原盘藻酸”，其比旋光度[α]22-D为 -83.6°，并对其理化性质进行了研究。经区带电泳、Sepharose 4B凝胶过滤和超速离心分析表明，纯化后的原盘藻酸呈均一状态。沉降系数为1.8S，特性粘度为1.1dl/g。通过凝胶过滤法估计其分子量约为170000。高碘酸盐氧化、甲基化分析和部分酸水解的结果表明，这种酸性多糖具有主要由1,4-连接构成的线性结构，并含有一个对酸不稳定的连接键。还描述了还原型原盘藻酸，其比旋光度[α]22-D为 -44°。
