Dorrbecker B R, Mercik S H, Kramer P A
J Chromatogr. 1984 Dec 12;336(2):293-300. doi: 10.1016/s0378-4347(00)85152-x.
A high-performance liquid chromatographic procedure is reported for reproducibly and sensitively quantitating caffeine and its N-demethylated metabolite paraxanthine in microsamples. A 5-micron reversed-phase radial compression column and 214-nm fixed wavelength ultraviolet detector were used to attain a sensitivity sufficient to quantitate these compounds at concentrations as low as 80 ng/ml using only 25 microliter of sample. The assay is applicable to microliter samples of whole blood, serum, plasma, saliva, amniotic, cerebrospinal and gastric fluids such as might be obtained in studies involving small animals or neonates. The utility of the assay is illustrated with caffeine and paraxanthine levels measured in several maternal and fetal fluids following constant-rate intravenous infusion of caffeine into a rabbit throughout pregnancy.
报道了一种高效液相色谱法，可用于对微量样品中的咖啡因及其N-去甲基代谢物副黄嘌呤进行可重复且灵敏的定量分析。使用5微米反相径向压缩柱和214纳米固定波长紫外检测器，仅用25微升样品就能达到足够的灵敏度，以定量低至80纳克/毫升浓度的这些化合物。该测定法适用于全血、血清、血浆、唾液、羊水、脑脊液和胃液的微升样品，例如在涉及小动物或新生儿的研究中可能获得的样品。通过在整个孕期对兔子进行恒速静脉输注咖啡因后，测量几种母体和胎儿体液中的咖啡因和副黄嘌呤水平，说明了该测定法的实用性。
